Self-Assembled Copolymeric Nanowires as a New Class of 3D Scaffold for Stem Cells Growth and Proliferation

Stem cell therapy has emerged as the most vibrant area of research, due to capacity stem cells for self-renewal and differentiation into different types lines upon their culture. But lately, scientists become increasingly aware limitations conventional 2D culture media, several key drawbacks associated with this model, such immune response transplantation, animal pathogen contamination, complication, during developmental studies undefined factors in cultural media. In study, an attempt been made develop a new type polymeric 3D scaffold based on self-assembly star-like amphiphilic copolymer poly(caprolactone)–poly(ethylene oxide) unit nanowires (nanofibers), that have scale similar native extracellular matrix are capable mimicking microenvironment where functional properties can be observed manipulated. The obtained data showed polymeric-based nanofibers used mouse embryonic (mESCs) growth without losing phenotype. results suggest scaffolds (nanofibers) not only support cells’ proliferation but also preserve mESC pluripotency.